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山羊 anti-小鼠 IgM (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
产品编号 ABIN102674
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 191
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    小鼠
    宿主
    • 320
    • 188
    • 75
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    山羊
    克隆类型
    • 495
    • 80
    多克隆
    应用范围
    • 334
    • 249
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    • 144
    • 139
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    • 1
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgM µ chain
    交叉反应
    小鼠
    产品特性
    Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Mouse IgM whole molecule

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Mouse IgM (mu chain) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,400 - 1:40,400

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mouse (mu chain) antibody, Mouse IgM (mu chain) Antibody in goat, Goat anti-mouse IgM Secondary Antibody

    Background: Anti-Mouse IgM (mu chain) antibody generated in goat detects specifically mouse IgM (mu chain). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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