兔 anti-小鼠 IgG2a (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- 抗原 See all IgG2a products
- IgG2a
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG2a
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Mouse IgG2a heavy chain
- 亚型
- IgG
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Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Mouse IgG2a (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rat anti-Mouse IgG2a antibodyELISA, FACS Monoclonal SB84a IgG1 unconjugated
Goat anti-Mouse IgG2a (Heavy Chain) antibody (FITC) - PreadsorbedFACS, IHC, WB, FLISA, FM Polyclonal IgG FITC
Rat anti-Mouse IgG2a antibody (PE)ELISA, FACS Monoclonal SB84a IgG1 PE
Rat anti-Mouse IgG2a antibody (HRP)ELISA, IHC Monoclonal SB84a IgG1 HRP
Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgG2a antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgG2a antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgG2a antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody alkaline phosphatase Conjugated, Rabbit Anti-Mouse IgG2a Antibody alk phos Conjugation
Background: Anti-Mouse IgG2a Alkaline Phosphatase antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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