兔 anti-绵羊 IgG (Fc Region) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Fc Region
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG F(c)
- 交叉反应
- 绵羊
- 产品特性
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Anti-Sheep IgG F(c) antibody generated in rabbit detects specifically sheep IgG F(c). Anti-Sheep IgG F(c) secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: immunoaffinity chromatography using Sheep IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB, LF Polyclonal IgG unconjugated
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)ELISA, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Anti-Sheep IgG F(c) antibody has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:10,000 - 1:200,000
Western Blot Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
Background: Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
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