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山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
产品编号 ABIN102117
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
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    Heavy & Light Chain
    适用
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    大鼠
    宿主
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    山羊
    克隆类型
    • 3346
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    产品特性
    Anti-RAT IgG (H&L) (GOAT) Antibody generated in sheep detects specifically rat IgG whole molecule. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads
    过滤
    Sterile filtered
    免疫原

    Immunogen: Rat IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: Anti-RAT IgG (H&L) (GOAT) Antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.

    ELISA Dilution: 1:20,000-1:100,000

    Western Blot Dilution: 1:2,000-1:10,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    有效期
    12 months
  • Scekic-Zahirovic, Oussini, Mersmann, Drenner, Wagner, Sun, Allmeroth, Dieterlé, Sinniger, Dirrig-Grosch, René, Dormann, Haass, Ludolph, Lagier-Tourenne, Storkebaum, Dupuis: "Motor neuron intrinsic and extrinsic mechanisms contribute to the pathogenesis of FUS-associated amyotrophic lateral sclerosis." in: Acta neuropathologica, Vol. 133, Issue 6, pp. 887-906, (2017) (PubMed).

    Zhang, Fu, Chen, Ren, Qu, Zhang, Yao, Yang: "Lacidipine attenuates apoptosis via a caspase-3 dependent pathway in human kidney cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 4, pp. 1040-9, (2014) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat anti-rat IgG, Rat antibody, rat antibodies, immunoglobulin

    Background: Anti-RAT IgG (H&L) (GOAT) Antibody generated in goat detects specifically rat IgG whole molecule. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.

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