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山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
产品编号 ABIN101985
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
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    • 15
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 692
    • 672
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    • 1
    • 1
    • 1
    宿主
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    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
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    Alkaline Phosphatase (AP)
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
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    • 115
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    交叉反应
    产品特性
    Anti-Rabbit IgG alkaline phosphatase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary alkaline phosphatase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-Rabbit IgG (H&L) alkaline phosphatase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:3,000 - 1:25,000

    Western Blot Dilution: 1:1,000 - 1:4,000

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, goat anti-Rabbit IgG Alk Phos Conjugated Antibody, goat anti-rabbit IgG heavy and light chain Antibody conjugated to Alkaline phosphatase

    Background: Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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