山羊 anti-人 IgG (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (gamma chain)
- 产品特性
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Anti-Human IgG (gamma chain) Antibody generated in Goat detects specifically human IgG gamma heavy chain. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer. and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm - 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Human IgG gamma heavy chain
- 亚型
- IgG
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Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (F(ab')2 Region) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Mouse anti-Human IgG (Heavy & Light Chain) antibodyELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated
Goat anti-Human IgG (Fc Region) antibody (HRP)ELISA, WB Polyclonal HRP F(ab')2 fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Human IgG Alkaline Phosphatase is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA Dilution: 1:4,000 - 1:16,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Human IgG (gamma chain) alkaline phosphatase conjugated Antibody, goat anti-Human IgG Antibody alk phos conjugation
Background: Anti-Human IgG (gamma chain) Alkaline Phosphatase generated in goat detects human Immunoglobulin G (gamma chain). It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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