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小鸡 anti-人 IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Texas Red (TR)
产品编号 ABIN101473
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 692
    • 671
    • 559
    • 452
    • 366
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    • 3
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    • 3
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    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
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    • 33
    • 31
    • 21
    • 17
    • 15
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    • 11
    • 1
    小鸡
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
    • 556
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    • 10
    Texas Red (TR)
    应用范围
    • 1837
    • 1789
    • 1786
    • 1499
    • 962
    • 933
    • 912
    • 515
    • 234
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    • 100
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Human antigens coupled to agarose beads
    免疫原

    Immunogen: Human IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    4.1
  • 应用备注

    Application Note: Anti-Human IgG (H&L) Texas Red is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Texas Red™ is a registered trademark of Molecular Probes Inc.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Chicken Anti Human IgG Antibody Texas Red™ Conjugated, Chicken Anti-Human IgG Antibody Texas Red™ Conjugated

    Background: Anti-Human IgG (H&L) Texas Red generated in chicken detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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