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山羊 anti-马 IgG (Fc Region) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN101446
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
    • 498
    • 411
    • 188
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    • 122
    • 92
    • 29
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    • 15
    • 9
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    适用
    • 692
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    • 3
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
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    • 33
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    • 15
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    山羊
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
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    Biotin
    应用范围
    • 1836
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    • 515
    • 234
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    • 2
    • 1
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG F(c)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Horse IgG F(c) fragment

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product has been assayed against 1.0 μg of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Thimerosal

    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat Anti-Horse IgG F(c) Antibody Biotin Conjugated, Goat Anti Horse IgG Fc Fragment Antibody Biotin Conjugated, Goat Anti Horse IgG Fc Antibody Biotin Conjugated

    Background: Anti-Horse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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