山羊 anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- Armenian Hamster
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG (H&L)
- 交叉反应
- Armenian Hamster
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Armenian Hamster IgG whole molecule
- 亚型
- IgG
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Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB IgG unconjugated
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Goat anti-Armenian Hamster IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Hamster Secondary Antibody conjugated to alkaline phosphatase is available in a variety of formats. Anti-IgG Secondary Antibody is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:40,000
Western Blot Dilution: 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: goat anti-Armenian Hamster IgG Antibody alkaline phosphatase Conjugation, goat anti-Armenian Hamster IgG alkaline phosphatase Conjugated Antibody, goat Anti-Hamster IgG alk phos Conjugated Secondary Antibody
Background: Anti-Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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