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兔 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG FITC
产品编号 ABIN101298
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
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    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 692
    • 672
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    • 2
    • 1
    • 1
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    • 1
    豚鼠
    宿主
    • 1718
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    • 103
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    克隆类型
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    • 1
    多克隆
    标记
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    FITC
    应用范围
    • 1837
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    • 515
    • 234
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    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
    免疫原

    Immunogen: Guinea Pig IgG whole molecule

    亚型
    IgG
    Labeling Ratio
    2.0
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.2 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Thimerosal

    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit Anti-Guinea Pig IgG Antibody Fluorescein Conjugated, rabbit Anti-Guinea Pig IgG Antibody FITC Conjugated

    Background: Anti-Guinea Pig IgG Fluorescein Antibody generated in rabbit detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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