山羊 anti-豚鼠 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with guinea pig whole molecule in goat.

Immunogen Type: Native Protein

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Guinea Pig (H&L) biotin conjugated antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary biotin conjugated antibody anti-Guinea Pig is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Antibody anti guinea pig biotin conjugated has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Synonyms: Guinea pig IgG (H&L) Antibody, Gt-a-Guinea pig Biotin conjugated, Guinea pig IgG (H&L) Antibody in goat, Goat IgG (H&L) Biotin conjugated Secondary Antibody.

Background: Anti-Guinea Pig IgG Biotin Antibody generated in goat detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.