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兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody (HRP)

ELISA, IHC, WB Polyclonal IgG HRP
产品编号 ABIN101039
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 692
    • 672
    • 559
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    • 366
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鸡
    宿主
    • 1718
    • 1473
    • 537
    • 203
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    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
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    • 11
    • 1
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
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    HRP
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
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    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG (H&L)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
    免疫原

    Immunogen: Chicken IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 μg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:46,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:1,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10.0 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: None

    储存液
    Without preservative
    注意事项
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti-Chicken IgG Antibody Peroxidase Conjugated, rabbit anti-Chicken IgG HRP Conjugated Antibody

    Background: Anti-Chicken IgG Peroxidase Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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