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兔 anti-Cow IgG (Fc Region) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN100940
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
    • 2333
    • 498
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
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    • 15
    • 9
    • 6
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    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    适用
    • 692
    • 672
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    • 2
    • 1
    • 1
    • 1
    • 1
    Cow
    宿主
    • 1718
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    • 33
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    • 17
    • 15
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    • 1
    克隆类型
    • 3346
    • 164
    • 1
    多克隆
    标记
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    Biotin
    应用范围
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
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    • 100
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    • 1
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG F(c)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: immunoaffinity chromatography using Bovine IgG coupled to agarose beads
    免疫原

    Immunogen: Bovine IgG F(c) fragment

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:15,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.5 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: rabbit anti Bovine IgG F(c) Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc Antibody Biotin Conjugated, rabbit anti Bovine IgG Fc fragment biotin conjugate

    Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

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