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山羊 anti-小鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
产品编号 ABIN100805
发货至: 中国
  • 抗原 See all IgA, IgG, IgM products
    IgA, IgG, IgM
    抗原表位
    • 74
    • 58
    • 31
    • 16
    • 9
    Heavy & Light Chain
    适用
    • 72
    • 46
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    小鼠
    宿主
    • 160
    • 55
    • 4
    • 3
    • 1
    山羊
    克隆类型
    • 218
    • 1
    多克隆
    标记
    • 54
    • 39
    • 33
    • 26
    • 22
    • 11
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 116
    • 111
    • 76
    • 73
    • 68
    • 56
    • 26
    • 24
    • 23
    • 22
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgG IgA IgM
    交叉反应
    小鼠
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Mouse IgG IgA and IgM whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

    ELISA Dilution: 1:500,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    2.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    有效期
    12 months
  • 抗原
    IgA, IgG, IgM
    别名
    IgG IgA IgM (IgA, IgG, IgM 产品)
    物质类
    Antibody
    背景

    Synonyms: Goat Anti Mouse IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Mouse IgG IgA IgM Biotin conjugated Antibody, Goat Anti-Mouse IgGAM Biotin conjugate

    Background: Anti-Mouse IgG IgA IgM Biotin Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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