山羊 anti-人 IgA, IgG, IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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- 抗原 See all IgA, IgG, IgM products
- IgA, IgG, IgM
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG IgA IgM
- 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Human IgG, IgA and IgM whole molecule
- 亚型
- IgG
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Human IgG IgA IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:3,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- IgA, IgG, IgM
- 别名
- IgG IgA IgM (IgA, IgG, IgM 产品)
- 物质类
- Antibody
- 背景
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Synonyms: Goat Anti-Human IgG IgA IgM Antibody alkaline phosphatase Conjugated, Goat Anti-Human IgG IgA IgM Antibody alk phos Conjugated, Goat Anti-Human IgGAM Antibody ALP Conjugated
Background: Anti-Human IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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