RHEB 抗体 (Ras Homolog Enriched in Brain) (Internal Region)

Details for Product anti-RHEB Antibody No. ABIN1003087
抗原
抗原表位
Internal Region
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适用
人, 小鼠, 大鼠
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宿主

102
9
克隆类型
多克隆
标记
This RHEB antibody is un-conjugated
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应用范围
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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选项
免疫原 Rabbit polyclol Rheb antibody was raised against a 15 amino acid peptide from the middle region of human Rheb (Genbank accession No. AAH16155).
Blocking Peptide Blocking peptide for this product available: ABIN1004040
亚型 IgG
纯化方法 Affinity chromatography purified via peptide column.
抗原
别名 Rheb (Ras Homolog Enriched in Brain) (RHEB Antibody 摘要)
背景 Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
途径 RTK signaling
应用备注 Rheb antibody can be used for the detection of Rheb by Western blot at 1 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
限制 仅限研究用
状态 Liquid
缓冲液 Antibody is supplied in PBS containing 0.02% sodium azide.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
储存条件 4 °C
有效期 12 months
有引用在: Inoki, Li, Xu, Guan: "Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling." in: Genes & development, Vol. 17, Issue 15, pp. 1829-34, 2003 (PubMed).

Stocker, Radimerski, Schindelholz, Wittwer, Belawat, Daram, Breuer, Thomas, Hafen: "Rheb is an essential regulator of S6K in controlling cell growth in Drosophila." in: Nature cell biology, Vol. 5, Issue 6, pp. 559-65, 2003 (PubMed).

Yee, Worley: "Rheb interacts with Raf-1 kinase and may function to integrate growth factor- and protein kinase A-dependent signals." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 921-33, 1997 (PubMed).