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GFP 抗体 (AA 246) (HRP)

Name: GFP 抗体 (AA 246) (HRP)
Brand: antibodies-online
SKU: ABIN100089
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal HRP

Western Blot of Anti-GFP Antibody Peroxidase Conjugate.

Western Blot of Goat anti-GFP antibody Peroxidase conjugated.

2 images

(11 references)

产品编号 ABIN100089

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
15

15

10

9

5

4

3

3

2

2

2

2

1

1

1

1

1
AA 246
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

34

11

9

6

2

1
山羊
Compatible Secondaries
克隆类型
120

85
多克隆
标记
104

14

12

8

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
应用范围
172

86

55

42

33

31

29

20

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

亚型

IgG

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC

应用备注

Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

限制

仅限研究用
状态

Lyophilized

溶解方式

Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Gentamicin sulfate

注意事项

Do NOT add Sodium Azide!

储存条件

4 °C
Hennigan, Fletcher, Guard, Ratner: "Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins." in: Science signaling, Vol. 12, Issue 578, (2020) (PubMed).

Lim, Chang, Huh: "Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).

Bisgrove, Su, Yost: "Maternal Gdf3 is an obligatory cofactor in Nodal signaling for embryonic axis formation in zebrafish." in: eLife, Vol. 6, (2018) (PubMed).

Botto, Totonchy, Gustin, Moses: "Kaposi Sarcoma Herpesvirus Induces HO-1 during De Novo Infection of Endothelial Cells via Viral miRNA-Dependent and -Independent Mechanisms." in: mBio, Vol. 6, Issue 3, pp. e00668, (2016) (PubMed).

Fahrenkamp, de Leur, Küster, Chatain, Müller-Newen: "Src family kinases interfere with dimerization of STAT5A through a phosphotyrosine-SH2 domain interaction." in: Cell communication and signaling : CCS, Vol. 13, pp. 10, (2015) (PubMed).

Gustin, Bai, Moses, Douglas: "Ebola Virus Glycoprotein Promotes Enhanced Viral Egress by Preventing Ebola VP40 From Associating With the Host Restriction Factor BST2/Tetherin." in: The Journal of infectious diseases, Vol. 212 Suppl 2, pp. S181-90, (2015) (PubMed).

Bejarano, Yuste, Patel, Stout, Spray, Cuervo: "Connexins modulate autophagosome biogenesis." in: Nature cell biology, Vol. 16, Issue 5, pp. 401-14, (2014) (PubMed).

Sung, Lim, Yi, Chang, Yang, Lee, Huh: "Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast." in: Genome research, Vol. 23, Issue 4, pp. 736-46, (2013) (PubMed).

Chatain, Ziegler, Fahrenkamp, Jost, Moriggl, Schmitz-Van de Leur, Müller-Newen: "Src family kinases mediate cytoplasmic retention of activated STAT5 in BCR-ABL-positive cells." in: Oncogene, (2012) (PubMed).

Ha, Sung, Huh: "Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae." in: Nucleic acids research, Vol. 40, Issue 11, pp. 4892-903, (2012) (PubMed).

Guarino, Shepherd, Salguero, Hua, Deegan, Kearsey: "Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation." in: Nucleic acids research, Vol. 39, Issue 14, pp. 5978-90, (2011) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

HRP Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.