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GFP 抗体 (AA 246) (Alkaline Phosphatase (AP))

Name: GFP 抗体 (AA 246) (Alkaline Phosphatase (AP))
Brand: antibodies-online
SKU: ABIN100086
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)

Western Blot of Alkaline Phosphatase conjugated Goat anti-GFP antibody.

Dot Blot of GFP Antibody Alkaline Phosphatase Conjugated.

2 images

产品编号 ABIN100086

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
15

15

10

9

5

4

3

3

2

2

2

2

1

1

1

1

1
AA 246
适用
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

34

11

9

6

2

1
山羊
Compatible Secondaries
克隆类型
120

85
多克隆
标记
104

14

12

9

6

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Alkaline Phosphatase (AP)
应用范围
172

86

55

42

33

31

29

20

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

产品特性

Concentration Definition: by UV absorbance at 280 nm

过滤

Sterile filtered

免疫原

The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

亚型

IgG

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应用备注

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Conjugated GFP antibody is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: ALP, Alk Phos, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.