ANXA2P2 Protein (AA 2-339) (His tag)
Crystallography grade
ANXA2P2
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Crys, ELISA, SDS, WB
2 images
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- 抗原
- ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-339
- 宿主
- 人
- 资源
- 大肠杆菌（E. Coli）
- 标记
- This ANXA2P2 protein is labelled with His tag.
- 应用范围
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
- STVHEILCKL SLEGDHSTPP SAYGSVKAYT NFDAERDALN IETAIKTKGV DEVTIVNIVT NRDNAQRQDI VFSYQRRTKK ELASALKSAL SGHLETVILG LLKTPAQYDA SELKASMKGL GTDEDSLIEI ICSRTNQELQ EINRVYKEMY KTDLEKDIIS DTSGDFRKLM VALAKGRRAE DGSVIDYELI DQDAQDLYDA GVKRKGTDVP KWISIMTERS VPHLQKVFDR YKSYSPYDML ESIRKEVKGD LENAFLNLVQ RIQNKPLYFA DQLYDSMKGK GTRDKVLIRI MVSRSEVDML KIRSEFKRKY GKSLYYYIQQ DTKGDYQKAL LYLCGGDD
- 产品特性
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This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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N-terminal His-tag
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 50 mM Hepes, pH 7.8; 300 mM NaCl, 5 % glycerin
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- ANXA2P2 (Annexin A2 Pseudogene 2 (ANXA2P2))
- 别名
- ANXA2P2
- 别名
- ANX2L2 Protein, ANX2P2 Protein, LPC2B Protein, annexin A2 pseudogene 2 Protein, ANXA2P2 Protein
- 背景
- Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. {ECO:0000250}.
- 分子量
- 39.5 kDa Including tag.
- UniProt
- A6NMY6
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