anti-大鼠 STAT5A 抗体 for Intracellular Staining

Recommended STAT5A Antibody (supplied by: Log in to see )

抗原
Signal Transducer and Activator of Transcription 5A (STAT5A) 抗体
  • MGF
  • STAT5
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
  • STAT5A
  • stat5
  • stat5b
  • stat5a
  • signal transducer and activator of transcription 5A
  • signal transducer and activator of transcription 5a
  • signal transducer and activator of transcription 5B
  • signal transducer and activator of transcription 5B L homeolog
  • STAT5A
  • Stat5a
  • stat5a
  • STAT5B
  • LOC100348518
  • LOC100716811
  • stat5b.L
抗原表位
pTyr694
101
54
47
22
20
16
10
8
6
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠, 大鼠, 绵羊
499
228
196
35
23
19
17
17
7
4
4
1
1
1
1
1
1
宿主
小鼠
355
148
16
克隆类型
单克隆
标记
This STAT5A antibody is conjugated to PerCP-Cy5.5
13
12
12
9
8
8
8
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
应用范围
Intracellular Staining (ICS)
399
235
170
97
92
90
47
42
26
17
10
9
7
7
6
4
3
2
2
1
1
1
1
1
1
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产品编号 ABIN1177246
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177254 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 8
1 ABIN1177250 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177249 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177248 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177251 ICS Pacific Blue Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177247 ICS PE-Cy7 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 4
1 ABIN1177252 ICS PE Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 0
1 ABIN1177253 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr694 Log in to see 47-Stat5 0

General

抗原 Signal Transducer and Activator of Transcription 5A (STAT5A) 抗体
抗原表位 pTyr694
(101), (54), (47), (22), (20), (16), (10), (8), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠, 绵羊
(499), (228), (196), (35), (23), (19), (17), (17), (7), (4), (4), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(355), (148), (16)
克隆类型 (克隆位点)
单克隆   ( )
标记 This STAT5A antibody is conjugated to PerCP-Cy5.5
(13), (12), (12), (9), (8), (8), (8), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Intracellular Staining (ICS)
(399), (235), (170), (97), (92), (90), (47), (42), (26), (17), (10), (9), (7), (7), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9个引用可参考
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STAT5A抗体详情

STAT5A目标详情 使用细节 贮存及处理 References for anti-STAT5A antibody (ABIN1177246) 图像
品牌 BD Phosflow™
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Phosphorylated Human Phosphorylated Stat5 Peptide
克隆位点 47-Stat5
亚型 IgG1, kappa

STAT5A目标详情

STAT5A抗体详情 使用细节 贮存及处理 References for anti-STAT5A antibody (ABIN1177246) 图像 回页首
抗原
别名 Stat5 (STAT5A Antibody 摘要)
背景 Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.
途径 JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation

使用细节

STAT5A抗体详情 STAT5A目标详情 贮存及处理 References for anti-STAT5A antibody (ABIN1177246) 图像 回页首
应用备注 This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I).
样本量 20 μL
限制 仅限研究用

贮存及处理

STAT5A抗体详情 STAT5A目标详情 使用细节 References for anti-STAT5A antibody (ABIN1177246) 图像 回页首
状态 Liquid
缓冲液 Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed.

References for anti-STAT5A antibody (ABIN1177246)

STAT5A抗体详情 STAT5A目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Johnston, Choi, Diamond, Yang, Crotty: "STAT5 is a potent negative regulator of TFH cell differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 2, pp. 243-50, 2012

Suni, Maino: "Flow cytometric analysis of cell signaling proteins." in: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, 2011

Prlic, Bevan: "Exploring regulatory mechanisms of CD8+ T cell contraction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 43, pp. 16689-94, 2008

Riou, Yassine-Diab, Van grevenynghe, Somogyi, Greller, Gagnon, Gimmig, Wilkinson, Shi, Cameron, Campos-Gonzalez, Balderas, Kelvin, Sekaly, Haddad: "Convergence of TCR and cytokine signaling leads to FOXO3a phosphorylation and drives the survival of CD4+ central memory T cells." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 79-91, 2007

Van De Wiele, Marino, Murray, Vo, Whetsell, Teague: "Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4235-44, 2004

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000

Imada, Leonard: "The Jak-STAT pathway." in: Molecular immunology, Vol. 37, Issue 1-2, pp. 1-11, 2000

Liu, Gaffen, Goldsmith: "JAK/STAT signaling by cytokine receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 271-8, 1998

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994