Citrate Synthase 蛋白 (CS)

Human Citrate Synthase (CS) interaction partners

1. citrate synthase and ACSS1 have tumorigenic functions in hepatocellular carcinoma

2. Citrate synthase activity was lower in patients with heart failure with preserved ejection fraction compared to controls.

3. mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers.

4. METTL12 methylates CS on Lys-395, which is localized in the CS active site. Interestingly, the METTL12-mediated methylation inhibited CS activity and was blocked by the CS substrate oxaloacetate.

5. Data suggest that METTL12, localized in mitochondrial matrix, modifies/methylates Lys368 of citrate synthase in external surface region close to its catalytic site; addition/removal of methylation has no effect on citrate synthase activity; Lys368 occurs in highly conserved sequence of amino acid residues of citrate synthase. (METTL12 = methyltransferase-like protein 12)

6. Data suggest that downregulation of citrate synthase (CS) expression in 293T cells leads to low level of ATP production, excessive superoxide formation and cell apoptosis, which implies a possible mechanism for hearing loss in A/J mice.

7. Hence, we conclude that SIRT3 exhibits neuroprotection via deacetylating and increasing mitochondrial enzyme activities.

8. Paretic muscle in hemiparetic stroke survivors had lower CS concentration.

9. The citrate synthase knockdown cells exhibited severe defects in respiratory activity and marked decreases in ATP production, but great increases in glycolytic metabolism.

10. Citrate synthase (CS) is a direct RORalpha target gene and one mechanism by which RORalpha regulates lipid metabolism is via regulation of CS expression.

11. Responses of skeletal and cardiac muscles in CS activity and gene expression at 1 and 48 h after endurance training. Acute effect of exercise on training-induced elevation in CS activity in rat soleus but not ventricle muscles.

12. possible role of CS-specific autoantibodies in the pathomechanism of allograft vasculopathy

13. It is likely that enhanced citrate synthase activity contributes to the conversion of glucose to lipids in pancreatic cancer providing substrate for membrane lipids synthesis.

14. These results provide an important basis for the study of mitochondrial dysfunction due to aberrant CSa trafficking.

Pig (Porcine) Citrate Synthase (CS) interaction partners

1. In pig heart CS, both symmetric (suggested by crystallography) and asymmetric motions are features of the protein structure, and as a result the behaviour in solution is largely non-cooperative.

2. Thermostabilization of thermosensitive CS by small heat shock proteins is achieved by stabilization of the C- and N-terminae in the protruding thermosensitive softspot.

3. structure was refined to an R factor of 0.179 (R(free) = 0.222) using synchrotron data to a resolution of 1.78 A

Mouse (Murine) Citrate Synthase (CS) interaction partners

1. The CS activity decreased in the infarcted tissue of wild-type (WT) mice at day 1 post-MI (p<0.05), but this was not observed in the MMP-9 null mice, suggesting that MMP-9 deletion helps to maintain the mitochondrial activity post-MI

2. Multiple lines of evidence implicate this missense mutation (H55N) as the underlying cause of ahl4-related hearing loss, likely through its effects on mitochondrial adenosine trisphosphate (ATP) and free radical production in cochlear hair cells.

3. H55N polymorphism in Cs could be the underlying cause of low CS activity and its high affinity for substrates in A/J mice compared with other strains.

4. A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes without affecting development of heart failure with pressure overload.

Rabbit Citrate Synthase (CS) interaction partners

1. CS and SERCA activities were significantly higher in the pubococcygeus (Pc) compared with the ischiocavernosus/bulbospongiosus (Ic/Bs) pelvic floor muscles, whereas the ChAT activity of the Ic/Bs was higher than that of the Pc muscle.