anti-小鸡 ZAP70 抗体 for Immunofluorescence

Recommended ZAP70 Antibody (supplied by: Log in to see )

抗原
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) 抗体
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
抗原表位
AA 468-619
79
53
49
37
28
24
23
15
13
12
10
10
10
8
8
8
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
小鸡, 人, 小鼠, 大鼠
580
218
142
14
11
10
8
8
6
6
4
3
1
宿主
小鼠
315
242
16
8
3
克隆类型
单克隆
标记
This ZAP70 antibody is un-conjugated
31
24
20
19
13
9
9
7
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
318
172
169
161
158
82
52
48
23
7
5
5
4
4
4
3
3
2
2
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产品编号 ABIN967848
产品在该地区无法提供。
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967849 IF IP IHC WB Mouse IgG2a AA 468-619 Log in to see 29-ZAP70 Kinase 5

General

抗原 zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) 抗体
抗原表位 AA 468-619
(79), (53), (49), (37), (28), (24), (23), (15), (13), (12), (10), (10), (10), (8), (8), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 小鸡, 人, 小鼠, 大鼠
(580), (218), (142), (14), (11), (10), (8), (8), (6), (6), (4), (3), (1)
宿主 小鼠
(315), (242), (16), (8), (3)
克隆类型 (克隆位点)
单克隆   ( )
标记 This ZAP70 antibody is un-conjugated
(31), (24), (20), (19), (13), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1)
应用范围 Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(318), (172), (169), (161), (158), (82), (52), (48), (23), (7), (5), (5), (4), (4), (4), (3), (3), (2), (2)
Pubmed 5个引用可参考
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ZAP70抗体详情

ZAP70目标详情 使用细节 贮存及处理 References for anti-ZAP70 antibody (ABIN967848) 图像
交叉反应 小鼠, 大鼠, 小鸡
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Human ZAP-70 Kinase aa. 468-619
克隆位点 29-ZAP70 Kinase
亚型 IgG2a

ZAP70目标详情

ZAP70抗体详情 使用细节 贮存及处理 References for anti-ZAP70 antibody (ABIN967848) 图像 回页首
抗原
别名 ZAP-70 (ZAP70 Antibody 摘要)
背景 ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.
分子量 70 kDa
研究领域 Signaling, Tags/Labels, Immunology, Cancer
途径 TCR Signaling

使用细节

ZAP70抗体详情 ZAP70目标详情 贮存及处理 References for anti-ZAP70 antibody (ABIN967848) 图像 回页首
说明

Related Products: ABIN968537, ABIN967389

限制 仅限研究用

贮存及处理

ZAP70抗体详情 ZAP70目标详情 使用细节 References for anti-ZAP70 antibody (ABIN967848) 图像 回页首
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20°C.

References for anti-ZAP70 antibody (ABIN967848)

ZAP70抗体详情 ZAP70目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Michel, Attal-Bonnefoy, Mangino, Mise-Omata, Acuto: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001

Mora, Stanley, Armistead, Chan, Boothby: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001

Maccalli, Pisarra, Vegetti, Sensi, Parmiani, Anichini: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000

背景

Arpaia, Shahar, Dadi, Cohen, Roifman: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994

Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994

图像

ZAP70抗体详情 ZAP70目标详情 使用细节 贮存及处理 References for anti-ZAP70 antibody (ABIN967848) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 468-619) antibody (ABIN967848) Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia...