小鼠 MET ELISA Kit

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抗原
Met Proto-Oncogene (MET) ELISA试剂盒
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
适用
小鼠
其他选择
Kits with alternative reactivity to:
43
21
15
3
2
2
1
1
1
1
1
应用范围
ELISA
选项
Supplier
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产品编号 ABIN2114064
$ 525.97
加上运输费 $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN577769 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.078-5 ng/mL 0.078 ng/mL Log in to see
1 ABIN625228 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN625241 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN5563545 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.082-20 ng/mL 0.082 ng/mL Log in to see
1 ABIN1380640 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380641 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380228 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380392 Cell ELISA Cell Culture Cells Log in to see
1 ABIN625400 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 0.082-20 ng/mL 0.082 ng/mL Log in to see
1 ABIN1381312 Cell ELISA Cell Culture Cells Log in to see
1 ABIN773252 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
1 ABIN2684958 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see 1
1 ABIN2113239 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2113240 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2113238 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2113237 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2684957 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN2042367 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2684959 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN1136430 Log in to see

General

抗原 Met Proto-Oncogene (MET) ELISA试剂盒
适用 小鼠
Kits with alternative reactivity to:
(43), (21), (15), (3), (2), (2), (1), (1), (1), (1), (1)
应用范围 ELISA
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METELISA Kit详情

目标详细情况 使用细节 贮存及处理 图像
样品类型 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin)
检测方法 Colorimetric
交叉反应 (详细) No detectable cross-reactivity with any other cytokine.
灵敏度 < 5 pg/mL

目标详细情况

METELISA Kit详情 使用细节 贮存及处理 图像 回页首
抗原
别名 C-MET/HGFR (MET ELISA Kit 摘要)
背景 Synonyms: Hepatocyte growth factor receptor, MET, HGFR
Gene Symbol: MET
基因ID 17295
UniProt A4FUV6
途径 RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

使用细节

METELISA Kit详情 目标详细情况 贮存及处理 图像 回页首
实验流程
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
限制 仅限研究用

贮存及处理

METELISA Kit详情 目标详细情况 使用细节 图像 回页首
注意事项 Avoid multiple freeze-thaw cycles
储存条件 4 °C
储存方法 Store at 4 °C for frequent use, at -20°C for infrequent use.
有效期 6 months

图像

METELISA Kit详情 目标详细情况 使用细节 贮存及处理 回页首
厂商提供的图像
ELISA image for Met Proto-Oncogene (MET) ELISA Kit (ABIN2114064) Met Proto-Oncogene (MET) ELISA Kit