人 MET ELISA Kit

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抗原
Met Proto-Oncogene (MET) ELISA试剂盒
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
抗原表位
AA 25-307, AA 308-932
适用

其他选择
Kits with alternative reactivity to:
42
22
15
3
2
2
1
1
1
1
1
实验类型
Sandwich ELISA
检测范围
62.5-4000 pg/mL
最低检测浓度
62.5 pg/mL
应用范围
ELISA
选项
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产品编号 ABIN1672788
$ 400.00
加上运输费 $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.438807 ABIN417905 Sandwich ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 125 pg/mL - 8000 pg/mL 125 pg/mL Log in to see
10.438807 ABIN577767 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
10.438807 ABIN1570293 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
10.438807 ABIN2703084 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 60-15.000 pg/mL 60 pg/mL Log in to see
10.438807 ABIN2708173 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
10.438807 ABIN2114063 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
1 ABIN625228 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN625241 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN5595409 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN4993092 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 61.4-15000 pg/mL 61.4 pg/mL Log in to see
1 ABIN5518635 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN1380640 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380641 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380228 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1380392 Cell ELISA Cell Culture Cells Log in to see
1 ABIN1381312 Cell ELISA Cell Culture Cells Log in to see
1 ABIN4886207 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN5655085 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 1.25 ng/mL - 80 ng/mL 1.25 ng/mL Log in to see
1 ABIN509702 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
1 ABIN1884672 Sandwich ELISA Plasma, Serum, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see

General

抗原 Met Proto-Oncogene (MET) ELISA试剂盒
抗原表位 AA 25-307, AA 308-932
适用
Kits with alternative reactivity to:
(42), (22), (15), (3), (2), (2), (1), (1), (1), (1), (1)
实验类型 Sandwich ELISA
检测范围 62.5-4000 pg/mL
最低检测浓度 62.5 pg/mL
应用范围 ELISA
Pubmed 3个引用可参考
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METELISA Kit详情

目标详细情况 使用细节 贮存及处理 References for MET Kit (ABIN1672788) 图像
原理 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human C-MET/HGFR
品牌 PicoKine™
样品类型 Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
检测方法 Colorimetric
Analytical Method Quantitative
特异性 Expression system for standard: NSO
Immunogen sequence: E25-R307(alpha)&S308-T932(beta)
交叉反应 (详细) There is no detectable cross-reactivity with other relevant proteins.
灵敏度 <5pg/mL
试剂未包括 Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
免疫原 Expression system for standard: NSO
Immunogen sequence: E25-R307(alpha)&S308-T932(beta)

目标详细情况

METELISA Kit详情 使用细节 贮存及处理 References for MET Kit (ABIN1672788) 图像 回页首
抗原
别名 MET (MET ELISA Kit 摘要)
背景

Protein Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.

Background: C-Met(MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor(HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

Synonyms: Hepatocyte growth factor receptor,HGF receptor,2.7.10.1,HGF/SF receptor,Proto-oncogene c-Met,Scatter factor receptor,SF receptor,Tyrosine-protein kinase Met,MET,

Full Gene Name: Hepatocyte growth factor receptor

Cellular Localisation: Membrane, Single-pass type I membrane protein.
基因ID 4233
UniProt P08581
途径 RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

使用细节

METELISA Kit详情 目标详细情况 贮存及处理 References for MET Kit (ABIN1672788) 图像 回页首
应用备注 Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
说明

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.

Tissue Specificity: Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. .

板类型 Pre-coated
实验流程 human C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for C-MET has been precoated onto 96-well plates. Standards(NSO, E25-R307 (α) &S308-T932(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human C-MET amount of sample captured in plate.
实验流程 Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human C-MET standard solution and each sample be measured in duplicate.
实验精密度
  • Sample 1: n=16, Mean(ng/ml): 0.54, Standard deviation: 0.028, CV(%): 5.2
  • Sample 2: n=16, Mean(ng/ml): 1.51, Standard deviation: 0.077, CV(%): 5.1
  • Sample 3: n=16, Mean(ng/ml): 2.4, Standard deviation: 0.108, CV(%): 4.5,
  • Sample 1: n=24, Mean(ng/ml): 0.49, Standard deviation: 0.03, CV(%): 6.1
  • Sample 2: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.118, CV(%): 7.4
  • Sample 3: n=24, Mean(ng/ml): 2.6, Standard deviation: 0.174, CV(%): 6.7
限制 仅限研究用

贮存及处理

METELISA Kit详情 目标详细情况 使用细节 References for MET Kit (ABIN1672788) 图像 回页首
注意事项 Avoid multiple freeze-thaw cycles.
储存条件 -20 °C,4 °C
储存方法 Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
有效期 12 months

References for MET Kit (ABIN1672788)

METELISA Kit详情 目标详细情况 使用细节 贮存及处理 图像 回页首
背景

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." in: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, 2012

Galland, Stefanova, Lafage, Birnbaum: "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23." in: Cytogenetics and cell genetics, Vol. 60, Issue 2, pp. 114-6, 1992

Bottaro, Rubin, Faletto, Chan, Kmiecik, Vande Woude, Aaronson: "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product." in: Science (New York, N.Y.), Vol. 251, Issue 4995, pp. 802-4, 1991

图像

METELISA Kit详情 目标详细情况 使用细节 贮存及处理 References for MET Kit (ABIN1672788) 回页首
厂商提供的图像
ELISA image for Met Proto-Oncogene (MET) ELISA Kit (ABIN1672788) Human C-MET/HGFR PicoKine ELISA Kit standard curve