抗原 See all FAS 蛋白: FAS (TNF Receptor Superfamily, Member 6 (FAS))
蛋白类型: Recombinant
产品特性: Soluble
宿主 26 10 4 3 2 2 1 1 1 1: 人
资源 21 11 6 4 3 2 2 1: Insect Cells
应用范围: Western Blotting (WB), Standard (STD)
品牌: BD Pharmingen™
产品特性: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
纯化方法: Recombinant soluble human Fas was expressed in the Baculovirus Expression Vector System and purified from insect cell lysate using an anti-Fas antibody. This form of Fas lacks the transmembrane domain of the Fas receptor, and has been referred to as FasΔTM. It has a 63-base pair deletion starting at nucleotide sequence (700) GA TCC AGA and ending at nucleotide sequence GTT TGG G (762). The purity and the integrity of the protein were verified by SDS-PAGE, and ELISA. FasΔTM has a reduced molecular weight of ~40 kDa.
Top Product: Discover our top product FAS 蛋白

TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) protein (Fc Tag)

FAS 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

TNF Receptor Superfamily, Member 6 (FAS) (AA 22-169) protein (Fc Tag)

FAS 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) protein (His tag)

FAS 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

TNF Receptor Superfamily, Member 6 (FAS) (AA 25-169) protein (His-DYKDDDDK-Strep II Tag)

FAS 宿主: 人宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI

TNF Receptor Superfamily, Member 6 (FAS) protein (His tag)

FAS 宿主: 小鼠宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE

TNF Receptor Superfamily, Member 6 (FAS) (AA 17-173) protein (His tag)

FAS 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA

TNF Receptor Superfamily, Member 6 (FAS) (AA 1-335) protein (GST tag)

FAS 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AP, AA

TNF Receptor Superfamily, Member 6 (FAS) (AA 20-119) protein (GST tag)

FAS 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AP, AA

TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)

FAS 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active

TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)

FAS 宿主: 大鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active

应用备注: Purified FasDeltaTM can be used as a positive control for western blot analysis of Fas. 1 µg of protein can easily be visualized on a Commasie-stained gel as a band of ~40 kDa. For western blot analysis, 1 µg or less is sufficient depending on the detection method (less is needed for enhanced chemiluminescence than for standard alkaline phosphatase detection). Purified FasDeltaTM can also be used as a standard in Fas ELISA assays. The recombinant FasDeltaTM protein should not be used in functional assays, as it does not appear to assume a biologically active conformation.
说明: BD Pharmingen™ Recombinant Soluble Human Fas (FasδTM)
限制: 仅限研究用

状态: Liquid
注意事项: Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.
储存条件: -20 °C,-80 °C
储存方法: Avoid multiple freeze-thaws of product. Store the protein at -20°C, or for longer storage life, at -80°C.

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

Cheng, Zhou, Liu, Shapiro, Brauer, Kiefer, Barr, Mountz: "Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1759-62, (1994) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

抗原: FAS (TNF Receptor Superfamily, Member 6 (FAS))
别名: Fas (FAS 产品)
别名: ALPS1A Protein, APO-1 Protein, APT1 Protein, CD95 Protein, FAS1 Protein, FASTM Protein, TNFRSF6 Protein, AI196731 Protein, APO1 Protein, TNFR6 Protein, Tnfrsf6 Protein, lpr Protein, Fas cell surface death receptor Protein, Fas (TNF receptor superfamily member 6) Protein, FAS Protein, Fas Protein, fas Protein
背景: Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.
途径: p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

FAS Protein (Soluble)

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