Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) 蛋白

ABIN934919 产品详细信息, 供应商: Log in to see
蛋白名称
  • CSF2
  • GM-CSF
  • GMCSF
  • CSF
  • Csfgm
  • Gm-CSf
  • MGI-IGM
  • Gm-csf
  • Gmcsf
  • granulocyte-macrophage colony-stimulating-factor
  • colony stimulating factor 2 (granulocyte-macrophage)
  • granulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • GM-CSF
  • CSF2
  • Csf2
宿主

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资源
大肠杆菌(E. Coli)
53
9
8
5
4
3
2
2
2
2
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1
蛋白类型
Recombinant
生物活性
Active
应用范围
SDS-PAGE (SDS)
选项
Supplier
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序列 MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
产品特性 Purified recombinant Human GMCSF protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 0.18 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Molecular weight on SDS-PAGE will appear higher.
纯度 > 95 % pure
内毒素水平 < 1.0 EU per µg of protein (determined by LAL method)
背景 Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two disulfide bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: MGC131935 protein, GMCSF protein, Eosinophil Colony Stimulating Factor protein, CMCSF protein, Sargramostim protein, GM-CSF protein, Pluripoietin Alpha protein, Granulocyte Macrophage Colony Stimulating Factor protein, Colony-stimulating factor protein, , Colony Stimulating Factor 2 protein, CSF protein, Colony stimulating factor protein, CSF 2 protein, GM-CSF protein, CSF2 protein, CSF Alpha protein, Molgramostin protein, Granulocyte-macrophage colony-stimulating factor protein, MGC138897 protein, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity protein
分子量 14.6 kDa (128 AA)
研究领域 Cytokines, Growth Factors
途径 JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
应用备注 GM-CSF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
实验流程

Cell line: TF1 (human erythroleukemic cell).
Maintenance Condition: 10 % FBS RPMI 1640 with GM-CSF
Assay medium: 10 % FBS RPMI 1640 without Gm-CSF
Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL)
Starvation: 24 h,
Assay medium Incubation time: 24 hr (after sample treatment)
Concentration range: 0.048 ng/mL - 25 ng/mL
Detection method: MTT assay

限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Supplied as a liquid in PBS, pH 7.4.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 RT/-20 °C
储存方法 Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
厂商提供的图像
SDS-PAGE (SDS) image for Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (ABIN934919) Figure annotation denotes ug of protein loaded and % gel used.
有引用在: Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, 2003 (PubMed).

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