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VTCN1 Protein (AA 33-194) (His tag,AVI tag,Biotin)

VTCN1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func
产品编号 ABIN4949005
发货至: 中国
  • 抗原 See all VTCN1 蛋白
    VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
    蛋白类型
    Recombinant
    产品特性
    AA 33-194
    宿主
    • 26
    • 6
    • 6
    • 3
    • 2
    • 1
    资源
    • 26
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    标记
    This VTCN1 protein is labelled with His tag,AVI tag,Biotin.
    应用范围
    Functional Studies (Func)
    品牌
    MABSol®,PrecisionAvi
    序列
    AA 33-194
    特异性
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    产品特性
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.8 kDa. As a result of glycosylation, the protein migrates as 35-45 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
    纯度
    >95 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product VTCN1 蛋白
  • 说明

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4
    注意事项
    Please avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
  • Johnston, Su, Pinckney, Critton, Boyer, Krishnakumar, Corbett, Rankin, Dibella, Campbell, Martin, Lemar, Cayton, Huang, Deng, Nayeem, Chen, Ergel, Rizzo, Yamniuk, Dutta, Ngo, Shorts, Ramakrishnan et al.: "VISTA is an acidic pH-selective ligand for PSGL-1. ..." in: Nature, Vol. 574, Issue 7779, pp. 565-570, (2019) (PubMed).

  • 抗原
    VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
    别名
    B7-H5 (VTCN1 产品)
    别名
    B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein
    背景
    Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
    分子量
    20.8 kDa
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