WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
蛋白类型
Recombinant
生物活性
Active
产品特性
AA 19-352
宿主
人
资源
Mammalian Cells
应用范围
Functional Studies (Func)
特异性
Stem cell or organoid culture
纯化方法
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling
纯度
70-75 %
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg)
Biological Activity Comment
Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 22JAN2016) generate 50- fold increase of luciferase activity compared to control (buffer without Wnt3a).
31-60 ng/mL To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only.
限制
仅限研究用
状态
Liquid
缓冲液
Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
注意事项
Mix the protein by pipetting up and down but not by vortexing
储存条件
-20 °C
储存方法
Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
抗原
WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
WNT3A Protein, Wnt-3a Protein, vt Protein, Zwnt[a] Protein, wnt3 Protein, wnt3 l Protein, wnt3l Protein, wnt[a] Protein, Xwnt-3a Protein, wnt-3a Protein, wnt3a-A Protein, xwnt3a Protein, WNT-3A Protein, WNT3 Protein, wingless-type MMTV integration site family, member 3A Protein, Wnt family member 3A Protein, wingless-type MMTV integration site family member 3A L homeolog Protein, WNT3A Protein, Wnt3a Protein, wnt3a Protein, wnt3a.L Protein
背景
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96 % amino acid identity to mouse Wnt3a protein.
分子量
41 kDa evaluated by SDS-PAGE under reducing conditions