Checkpoint Kinase 1 (CHEK1) (Active) 蛋白

ABIN457519 产品详细信息, 供应商: Log in to see
蛋白名称
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
宿主

14
3
1
1
1
资源
Baculovirus infected Insect Cells
7
4
3
2
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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原理 >90% Pure active Recombinant CHK1.
产品特性 Protein Source: Baculovirus (Sf9 insect cells)
纯度 SDS-PAGE: ≥90 %
背景 CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2). In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases (3). CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.
Alternate Names: CHK, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1
分子量 82.0 kDa
基因ID 1111
UniProt O14757
研究领域 Kinases/Phosphatases, Cell Cycle
途径 p53 Pathway, Apoptosis, Cell Division Cycle
应用备注 Optimal working dilution should be determined by the investigator.
说明

Activity Specification: 369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

限制 仅限研究用
状态 Liquid
浓度 0.1 mg/mL
缓冲液 Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30 % glycerol.).
储存液 Dithiothreitol (DTT)
注意事项 This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Centrifuge the vial prior to opening.
储存条件 -80 °C
储存方法 -80°C
有效期 12 months
厂商提供的图像
Western Blotting (WB) image for Checkpoint Kinase 1 (CHEK1) (Active) 蛋白 (ABIN457519) Checkpoint Kinase 1 (CHEK1) (Active) protein
有引用在: Larsen, Rampalli, Burns, Brunette, Dilworth, Megeney: "Caspase 3/caspase-activated DNase promote cell differentiation by inducing DNA strand breaks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 9, pp. 4230-5, 2010 (PubMed).