PKAcb (Active) 蛋白

ABIN411951 产品详细信息, 供应商: Log in to see
蛋白名称
宿主

1
资源
Baculovirus infected Insect Cells
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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原理 >90% Pure active Recombinant PKAcb.
产品特性 Protein Source: Baculovirus (Sf9 insect cells)
纯度 SDS-PAGE: ≥90 %
背景 Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (PKA). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-α, C-β, and C-γ. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-α, RI-β, RII-α, and RII-β. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-β of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-β of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic cell hybrids (1) and Simard et al located it to 1p36.1 by in situ hybridization (2).
Alternate Names: PKA, cAMP-dependent protein kinase catalytic subunit alpha
分子量 65.0 kDa
基因ID 5567
NCBI登录号 NM_002731
应用备注 Optimal working dilution should be determined by the investigator.
说明

Activity Specification: 342 μmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

限制 仅限研究用
状态 Liquid
浓度 0.1 μg/μL
缓冲液 Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
储存液 Dithiothreitol (DTT)
注意事项 This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Centrifuge the vial prior to opening.
储存条件 -80 °C
储存方法 -80°C
有效期 12 months
厂商提供的图像
SDS-PAGE (SDS) image for PKAcb (Active) 蛋白 (ABIN411951) PKAcb (Active) protein
Activity Assay (AcA) image for PKAcb (Active) 蛋白 (ABIN411951) PKAcb (Active) protein (Image 2)
背景 Skålhegg, Huang, Su, Idzerda, McKnight, Burton: "Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 3, pp. 630-9, 2002 (PubMed).

Yasuda, Barth, Stellwagen, Malenka: "A developmental switch in the signaling cascades for LTP induction." in: Nature neuroscience, Vol. 6, Issue 1, pp. 15-6, 2002 (PubMed).

Taskén, Solberg, Zhao, Hansson, Jahnsen, Siciliano: "The gene encoding the catalytic subunit C alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1." in: Genomics, Vol. 36, Issue 3, pp. 535-8, 1997 (PubMed).