Hemopoietic Cell Kinase (HCK) (Active) 蛋白

ABIN411927 产品详细信息, 供应商: Log in to see
蛋白名称
  • laloo
  • HCK
  • MGC107870
  • JTK9
  • p59Hck
  • p61Hck
  • AI849071
  • Bmk
  • Hctk
  • p56Hck
  • hemopoietic cell kinase
  • tklal-a
  • HCK
  • hck
  • Hck
宿主

12
2
1
资源
Baculovirus infected Insect Cells
6
2
2
1
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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原理 >90% Pure active Recombinant HCK.
产品特性 Protein Source: Baculovirus (Sf9 insect cells)
纯度 SDS-PAGE: ≥90 %
背景 HCK, a protein-tyrosine kinase, belongs to SRC family members (1). Ziegler et al. found that expression of HCK may be limited to certain hemopoietic cells and is especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (2). Therefore, Quintrell et al. designated the gene HCK (pronounced 'hick') for hemopoietic cell kinase. They described the nucleotide sequence of a cDNA clone and the distribution of RNA transcribed from HCK among various hemopoietic cells. They assigned the HCK gene to 20q11-q12. Since this region is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, they suggested that damage to HCK may contribute to the pathogenesis of these conditions (3).
Alternate Names: HCK, Tyrosine-protein kinase, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck
分子量 57.0 kDa
基因ID 3055
途径 Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
应用备注 Optimal working dilution should be determined by the investigator.
说明

Activity Specification: 171 nmol phosphate incorporated into poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

限制 仅限研究用
状态 Liquid
浓度 0.1 μg/μL
缓冲液 Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
储存液 Dithiothreitol (DTT)
注意事项 This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Centrifuge the vial prior to opening.
储存条件 -80 °C
储存方法 -80°C
有效期 12 months
厂商提供的图像
SDS-PAGE (SDS) image for Hemopoietic Cell Kinase (HCK) (Active) 蛋白 (ABIN411927) Hemopoietic Cell Kinase (HCK) (Active) protein
背景 Piccardoni, Manarini, Federico, Bagoly, Pecce, Martelli, Piccoli, Totani, Cerletti, Evangelista: "SRC-dependent outside-in signalling is a key step in the process of autoregulation of beta2 integrins in polymorphonuclear cells." in: The Biochemical journal, Vol. 380, Issue Pt 1, pp. 57-65, 2004 (PubMed).

Ziegler, Marth, Lewis, Perlmutter: "Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2276-85, 1987 (PubMed).

Quintrell, Lebo, Varmus, Bishop, Pettenati, Le Beau, Diaz, Rowley: "Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2267-75, 1987 (PubMed).