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NUP107 Protein (AA 1-926) (Strep Tag)

Crystallography grade NUP107 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
产品编号 ABIN3136158
发货至: 中国
  • 抗原 See all NUP107 蛋白
    NUP107 (Nucleoporin 107kDa (NUP107))
    蛋白类型
    Recombinant
    产品特性
    AA 1-926
    宿主
    • 2
    • 1
    小鼠
    资源
    • 2
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This NUP107 protein is labelled with Strep Tag.
    应用范围
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    序列
    MDRSGFGGMS SPVIRDAEVT RTARKHSAHK RVLIQANQED NFGTATPRSQ IIPRTPSSFR QPFVTPSSRS LLRHPDISYI LGTEGRSPRH TQSSGYLGNL SMVTNLDDSN WAAAFSSQRL GLYTNTEHHS MTEDVNLSTV MLREDDPGEA ASMSMFSDFL HSFLKHSSTT VFDLVEEYEN ICGSQVNILS KIVSRATPGL QKFSKTASML WLLQQEMVTW RLLASLYRDR IQSSLEEENM FAIAGINASE KMVVETLFQR DSLVRQSQLV VDWLESIAKD EIGEFSDNIE FYAKSVYWEN TLHSLKQRQL LSHMGSTRPL VTELDPDAPI RQKLPLDDLD REDEVRLLKY LFTLIRAGMT EEAQRLCKRC GQAWRAATLE GWKLYHDPNV NGGTELEPVE GNPYRRIWKI SCWRMAEDEL FNKYERAIYA ALSGNLKQLL PVCDTWEDTV WAYFRVMVDS LVEQEIRTSV MTQDDSEELP REYMEANWTL EKVFEELQAT DKKRVLEENQ EHYHIVQKFL ILGDVDGLMD EFSKWLSKSG SSLPGHLLRF MTHLILFLRT LGLQTKEEVS IEVLKTYIQL LISEKHTSLI AFYTCHLPQD LAVAQYALFL EGVTEFEQRH QCLELAKEAD LDVATITKTV VENICKKDNG EFSHHDLAPS LDTGTTEEDR LKIDVIDWLV FDPAQRAEAL RQGNAIMRKF LALKKHEAAK EVFVKIPQDS IAEIYNQWEE QGMESPLPAE DDNAIREHLC IRAYLEAHET FNEWFKHMNS APQKPTLLSQ ATFTEKVAYE HREKKYEMDH NIWKGHLDAL TADVKEKMYN VLLFVDGGWM VDVREDAEDD PERTHQMVLL RKLCLPMLCF LLHTILHSTG QYQECLQLAD MVSSERHKLY LVFSKEELRK LLQKLRESSL MLLDQGLDPL GYEIQS
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product NUP107 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    NUP107 (Nucleoporin 107kDa (NUP107))
    别名
    Nup107 (NUP107 产品)
    别名
    zgc:152723 Protein, si:dkey-27k7.3 Protein, Afu1g10860 Protein, BcDNA:LD18761 Protein, CG6743 Protein, Dmel\\CG6743 Protein, Nup 107 Protein, Nup170 Protein, dmNup107 Protein, nup107 Protein, SPBC582.11c Protein, AW541137 Protein, C76801 Protein, NUP84 Protein, nucleophosmin/nucleoplasmin 3 Protein, nucleoporin 107 Protein, nuclear pore complex protein Nup107 Protein, Nuclear pore complex protein Nup107 Protein, nucleoporin 107kDa S homeolog Protein, nucleoporin 107kDa Protein, Nucleoporin 107kD Protein, nucleoporin Nup107 Protein, NPM3 Protein, NUP107 Protein, nup107 Protein, AFUA_1G10860 Protein, LOC5563712 Protein, CpipJ_CPIJ003997 Protein, BDBG_00235 Protein, nu107 Protein, nup107.S Protein, Nup107 Protein
    背景
    Nuclear pore complex protein Nup107 (107 kDa nucleoporin) (Nucleoporin Nup107),FUNCTION: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. Involved in nephrogenesis. {ECO:0000250|UniProtKB:P57740}.
    分子量
    106.7 kDa
    UniProt
    Q8BH74
    途径
    Protein targeting to Nucleus
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