ISG15 Protein (AA 2-155) (His tag)
-
- 抗原 See all ISG15 蛋白
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-155
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This ISG15 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
-
AWDLKVKMLG GNDFLVSVTN SMTVSELKKQ IAQKIGVPAF QQRLAHQTAV LQDGLTLSSL GLGPSSTVML VVQNCSEPLS ILVRNERGHS NIYEVFLTQT VDTLKKKVSQ REQVHEDQFW LSFEGRPMED KELLGEYGLK PQCTVIKHLR LRGG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Isg15 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ISG15 蛋白
-
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-157) protein (CaM)
ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 98 % by SDS - PAGE SDS
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) protein (GST tag)ISG15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-100) protein (GST tag)ISG15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag)ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (His tag)ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- 别名
- Isg15 (ISG15 产品)
- 别名
- G1P2 Protein, IFI15 Protein, IP17 Protein, UCRP Protein, hUCRP Protein, ISG15 Protein, g1p2 Protein, si:rp71-1c23.2 Protein, G1p2 Protein, IGI15 Protein, Irfp Protein, ISG17 Protein, ISG17/UCRP Protein, ISG15 ubiquitin-like modifier Protein, ISG15 Protein, isg15 Protein, Isg15 Protein
- 背景
- Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. {ECO:0000269|PubMed:16254333, ECO:0000269|PubMed:17222803, ECO:0000269|PubMed:17227866, ECO:0000269|PubMed:17289916, ECO:0000269|PubMed:18057259, ECO:0000269|PubMed:22022510, ECO:0000269|PubMed:22028657, ECO:0000269|PubMed:22859821}.
- 分子量
- 18.2 kDa Including tag.
- UniProt
- Q64339
-
