DGKA Protein (AA 1-730) (Strep Tag)
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- 抗原 See all DGKA 蛋白
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-730
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This DGKA protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY LGFEQFMKMY LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV YCYFTLLEGG RPEDKLEFTF KLYDMDRNGI LDSTEVEKII LQMMRVAEYL DWDVSELRPI LQEMMREMDQ DGSGSVSLDE WVRAGATTVP LLVLLGMDVT MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK YIVHDHCAMK AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT TDDTSLCTPE AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR QVFDLKDGPE PGLRFFKDVP QFRILVCGGD GTVGWVLETI DKANFATVPP VAVLPLGTGN DLARCLRWGR GYEGENLRKI LKDIELSKVV YLDRWFLEVI PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP EKFNSRMKNK LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE VVGIEGAIEM GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA PCTIKITHKN QMPMLMGPPS NSYNFFGFWS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product DGKA 蛋白
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Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 341-555) protein (His-IF2DI Tag)
DGKA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (GST tag)DGKA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) protein (Strep Tag)Crystallography grade DGKA 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Diacylglycerol Kinase, alpha 80kDa (DGKA) proteinDGKA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DGKA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DGKA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)DGKA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Diacylglycerol Kinase, alpha 80kDa (DGKA) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)DGKA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
- 别名
- Dgka (DGKA 产品)
- 别名
- DAGK Protein, DAGK1 Protein, DGK-alpha Protein, 80kDa Protein, AW146112 Protein, Dagk1 Protein, dgka Protein, zgc:136759 Protein, dagk Protein, dagk1 Protein, dgk-alpha Protein, diacylglycerol kinase alpha Protein, diacylglycerol kinase, alpha Protein, diacylglycerol kinase, alpha 80kDa Protein, diacylglycerol kinase, alpha a Protein, diacylglycerol kinase alpha S homeolog Protein, DGKA Protein, Dgka Protein, dgkaa Protein, Tsp_02164 Protein, dgka Protein, dgka.S Protein
- 背景
- Diacylglycerol kinase alpha (DAG kinase alpha) (EC 2.7.1.107) (EC 2.7.1.93) (80 kDa diacylglycerol kinase) (Diglyceride kinase alpha) (DGK-alpha),FUNCTION: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol. {ECO:0000250|UniProtKB:P23743}.
- 分子量
- 82.8 kDa
- UniProt
- O88673
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