Cation Channel, Sperm-Associated, gamma 2 (CATSPERG2) (AA 39-1145) protein (rho-1D4 tag)

ABIN3131075 产品详细信息, 供应商: Log in to see
蛋白名称
  • 1700067C01Rik
  • CATSPERG
  • catsper channel auxiliary subunit gamma 2
  • Catsperg2
产品特性
AA 39-1145
1
宿主
小鼠
1
资源
Insect Cells
1
蛋白类型
Recombinant
标记
rho-1D4 tag
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 EDALQHCTWL LVLNKFEKVG LHLSKDRFQD HEPIDTVAKV FQKLTDSPID PSENYLSFPY YLQINFSCPG QNIEELARKG HLMGMKPMVQ INYMYSVNFY RWEMENVQIL MEAAPMRSTG YCPAEAMCVL NWYTPMPFKN GSVVSSVDIY TNGIGPFVSK KRFYVNMNGF LKRDASGKSL FAIGYESLVL KSSHFRLSKS RPLWYTVNHA PVFILGGFYD EKSILFSDSN FQDYVLLELS IDSCWVGSFY CPILGFSATI HDAIATESTL FIRQNQLVYY FTGTYITLFD KSHGSSRWVR VLPSECIKRL CPVYASGNGS EYVLALTTGK NEGYIHIGTI TDGLVSFEMV PDGWSVCEKL PGKNCSIDWA TYIADERNLL LLVKIDSGQF YLVNFNTEFK TLNILYKIPE FIPEAKELDF LVLLDTVTYT NTPMTPKGLF FNTLNNMLYI WGNFILQSYN REEFIFLADF PKESTIKYMV NSFKGQMAVV TENEEIWYFL EGGYDVYQVV PSQGWETYHN LQKMQKSSFH SEDESLVSLF FEDGKLFQLV YLFDVGKERL VKRLLPVGTL MEYNLPKPFT VVNQGNYQAI SFTHTCPFKE IHLIDVPKKH HASRTESYVA LPPLVSESLG FHNNNTLAVY QGLVYYLLWL HSKYDKPYAD PVHDPTWRWW QHKTKDKDYF FYLFSNRLAA EGIYINMNAY QKLYNMSGDY GIPDLFFLDK GNWFTITVVL LSHQDTFTSS DSQGPTINVD KKLAIAVTIA DPECLSVTVT QDVLLNRNAV INKIKVIDKK RCSEQGMIGR NIKKTSMMLK VLGAPGNCIQ RTYLGGIIQG FKVVPIFIGC PPGKRLAFDV SYTIMHSEEI NKHYFDCVIK DAEMPCFLFR DLFQPFFLVQ DLVTGDSGSF LGSYVLKVVG GGRTLNTIRD YTEEEIFRYN SPLDTTNSLI WKTKVERTTE DKKFYIMSHE SPGVEWLCLE NSPCYDIIPQ SIYPPEFFFK LLVSNRGVDN STYCDYKLTF IVHIHGLPLS SKRTSFIVMV STSFFIALVV FYILFCLVWP HIVKAWVSLR WRINNIMASE SYYTYASSTA GFSLQSHSFE GPSRAGSKED NVQAKTA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Catsperg2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin-free.
质量等级 Crystallography grade
背景 Probably involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. {ECO:0000269|PubMed:19516020}.
分子量 128.2 kDa Including tag.
UniProt C6KI89
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Cation Channel, Sperm-Associated, gamma 2 (CATSPERG2) (AA 39-1145) protein (rho-1D4 tag) (ABIN3131075) „Crystallography Grade“ protein due to multi-step, protein-specific purification process