Olfactory Receptor 480 (Olfr480) (AA 1-312) protein (rho-1D4 tag)

ABIN3127107 产品详细信息, 供应商: Log in to see
蛋白名称
  • S25
  • Ors25
  • MOR204-32
  • olfactory receptor 480
  • Olfr480
  • Olr480
产品特性
AA 1-312
宿主
小鼠
资源
Insect Cells
蛋白类型
Recombinant
标记
rho-1D4 tag
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 MEPGNYTVVT EFILLGLTDD ITVSVILFVM FLIVYSVTLM GNLNIIVLIR TSPQLHTPMY LFLSHLAFLD IGYSSSVTPI MLRGFLRKGT FIPVAGCVAQ LCIVVAFGTS ESFLLASMAY DRYVAICSPL LYSTQMSSTV CILLVGTSYL GGWVNAWIFT GCSLNLSFCG PNKINHFFCD YSPLLKLSCS HDFSFEVIPA ISSGSIIVVT VFIIALSYVY ILVSILKMRS TEGRQKAFST CTSHLTAVTL FFGTITFIYV MPQSSYSTDQ NKVVSVFYTV VIPMLNPLIY SFRNKEVKEA MKKLIAKTHW WS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Olfr480 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin-free.
质量等级 Crystallography grade
背景 Probable odorant receptor, which recognizes only aliphatic alcohols, suggesting that it may convey a "woody" or "sweet" sour. {ECO:0000269|PubMed:11802173}.
分子量 35.9 kDa Including tag.
UniProt Q8VEZ0
研究领域 Neurology
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Olfactory Receptor 480 (Olfr480) (AA 1-312) protein (rho-1D4 tag) (ABIN3127107) „Crystallography Grade“ protein due to multi-step, protein-specific purification process