ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 1-304) protein (rho-1D4 tag)

ABIN3125369 产品详细信息, 供应商: Log in to see
蛋白名称
  • im:7146264
  • orai1
  • tmem142a
  • zgc:109721
  • orai2
  • ORAI1
  • LOC100218031
  • CRACM1
  • ORAT1
  • TMEM142A
  • D730049H07Rik
  • Tmem142a
  • orai-1
  • RGD1311873
  • tmem142b
  • ORAI calcium release-activated calcium modulator 1b
  • ORAI calcium release-activated calcium modulator 1
  • ORAI calcium release-activated calcium modulator 2
  • orai1b
  • orai1
  • ORAI1
  • Orai1
  • LOC100218031
  • orai2
产品特性
AA 1-304
1
宿主
小鼠
1
资源
Insect Cells
1
蛋白类型
Recombinant
标记
This ORAI1 protein is labelled with rho-1D4 tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
选项
Supplier
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序列 MHPEPAPPPS HSNPELPVSG GSSTSGSRRS RRRSGDGEPS GAPPLPPPPP AVSYPDWIGQ SYSEVMSLNE HSMQALSWRK LYLSRAKLKA SSRTSALLSG FAMVAMVEVQ LDTDHDYPPG LLIVFSACTT VLVAVHLFAL MISTCILPNI EAVSNVHNLN SVKESPHERM HRHIELAWAF STVIGTLLFL AEVVLLCWVK FLPLKRQAGQ PSPTKPPAES VIVANHSDSS GITPGEAAAI ASTAIMVPCG LVFIVFAVHF YRSLVSHKTD RQFQELNELA EFARLQDQLD HRGDHSLTPG THYA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Orai1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

纯化方法 Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin-free.
质量等级 Crystallography grade
背景 Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. {ECO:0000250|UniProtKB:Q96D31}.
分子量 34.2 kDa Including tag.
UniProt Q8BWG9
研究领域 Adaptive Immunity, Signaling
途径 TCR Signaling
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (AA 1-304) protein (rho-1D4 tag) (ABIN3125369) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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