BCL2-Associated X Protein (BAX) (AA 1-192) protein (rho-1D4 tag)

ABIN3121499 产品详细信息, 供应商: Log in to see
蛋白名称
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
产品特性
AA 1-192
3
2
2
1
1
1
1
1
宿主
小鼠
10
7
1
1
资源
Insect Cells
8
2
1
1
蛋白类型
Recombinant
标记
This BAX protein is labelled with rho-1D4 tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 MDGSGEQLGS GGPTSSEQIM KTGAFLLQGF IQDRAGRMAG ETPELTLEQP PQDASTKKLS ECLRRIGDEL DSNMELQRMI ADVDTDSPRE VFFRVAADMF ADGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLV WIQDQGGWEG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Bax Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin-free.
质量等级 Crystallography grade
背景 Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production. {ECO:0000269|PubMed:21060336, ECO:0000269|PubMed:8358790}.
分子量 22.6 kDa Including tag.
UniProt Q07813
研究领域 Apoptosis/Necrosis, Cancer
途径 p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for BCL2-Associated X Protein (BAX) (AA 1-192) protein (rho-1D4 tag) (ABIN3121499) „Crystallography Grade“ protein due to multi-step, protein-specific purification process