Corin, Serine Peptidase (CORIN) (AA 868-1113) protein (rho-1D4 tag)

ABIN3120429 产品详细信息, 供应商: Log in to see
蛋白名称
  • CORIN
  • CG2105
  • Dmel\\CG2105
  • SP75
  • LOC559109
  • AV273130
  • Lrp4
  • ATC2
  • CRN
  • PEE5
  • TMPRSS10
  • corin, serine peptidase
  • CG2105 gene product from transcript CG2105-RC
  • novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN)
  • corin
  • CORIN
  • Corin
  • CH1073-76A20.1
  • LOC100340894
产品特性
AA 868-1113
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
宿主
小鼠
15
8
2
资源
Insect Cells
13
10
1
1
蛋白类型
Recombinant
标记
This Corin protein is labelled with rho-1D4 tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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序列 RILGGRTSRP GRWPWQCSLQ SEPSGHICGC VLIAKKWVLT VAHCFEGRED ADVWKVVFGI NNLDHPSGFM QTRFVKTILL HPRYSRAVVD YDISVVELSD DINETSYVRP VCLPSPEEYL EPDTYCYITG WGHMGNKMPF KLQEGEVRII PLEQCQSYFD MKTITNRMIC AGYESGTVDS CMGDSGGPLV CERPGGQWTL FGLTSWGSVC FSKVLGPGVY SNVSYFVGWI ERQIYIQTFL QKKSQG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Corin Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

纯化方法 Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin-free.
质量等级 Crystallography grade
背景 Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. {ECO:0000269|PubMed:11884416, ECO:0000269|PubMed:15637153, ECO:0000269|PubMed:20613715, ECO:0000269|PubMed:22418978, ECO:0000269|PubMed:22437503}.
分子量 28.9 kDa Including tag.
UniProt Q9Z319
研究领域 Proteolysis / Ubiquitin, Wnt, Cardiovascular
途径 Regulation of Systemic Arterial Blood Pressure by Hormones
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Corin, Serine Peptidase (CORIN) (AA 868-1113) protein (rho-1D4 tag) (ABIN3120429) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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