KIT Protein (AA 26-976) (rho-1D4 tag)
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- 抗原 See all KIT 蛋白
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 蛋白类型
- Recombinant
- 产品特性
- AA 26-976
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宿主
- 人
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资源
- Insect Cells
- 标记
- This KIT protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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QPSVSPGEPS PPSIHPGKSD LIVRVGDEIR LLCTDPGFVK WTFEILDETN ENKQNEWITE KAEATNTGKY TCTNKHGLSN SIYVFVRDPA KLFLVDRSLY GKEDNDTLVR CPLTDPEVTN YSLKGCQGKP LPKDLRFIPD PKAGIMIKSV KRAYHRLCLH CSVDQEGKSV LSEKFILKVR PAFKAVPVVS VSKASYLLRE GEEFTVTCTI KDVSSSVYST WKRENSQTKL QEKYNSWHHG DFNYERQATL TISSARVNDS GVFMCYANNT FGSANVTTTL EVVDKGFINI FPMINTTVFV NDGENVDLIV EYEAFPKPEH QQWIYMNRTF TDKWEDYPKS ENESNIRYVS ELHLTRLKGT EGGTYTFLVS NSDVNAAIAF NVYVNTKPEI LTYDRLVNGM LQCVAAGFPE PTIDWYFCPG TEQRCSASVL PVDVQTLNSS GPPFGKLVVQ SSIDSSAFKH NGTVECKAYN DVGKTSAYFN FAFKGNNKEQ IHPHTLFTPL LIGFVIVAGM MCIIVMILTY KYLQKPMYEV QWKVVEEING NNYVYIDPTQ LPYDHKWEFP RNRLSFGKTL GAGAFGKVVE ATAYGLIKSD AAMTVAVKML KPSAHLTERE ALMSELKVLS YLGNHMNIVN LLGACTIGGP TLVITEYCCY GDLLNFLRRK RDSFICSKQE DHAEAALYKN LLHSKESSCS DSTNEYMDMK PGVSYVVPTK ADKRRSVRIG SYIERDVTPA IMEDDELALD LEDLLSFSYQ VAKGMAFLAS KNCIHRDLAA RNILLTHGRI TKICDFGLAR DIKNDSNYVV KGNARLPVKW MAPESIFNCV YTFESDVWSY GIFLWELFSL GSSPYPGMPV DSKFYKMIKE GFRMLSPEHA PAEMYDIMKT CWDADPLKRP TFKQIVQLIE KQISESTNHI YSNLANCSPN RQKPVVDHSV RINSVGSTAS SSQPLLVHDD V
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human KIT Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product KIT 蛋白
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Mast/stem Cell Growth Factor Receptor (KIT) protein (His tag)
KIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Mast/stem Cell Growth Factor Receptor (KIT) (Val560Gly-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Thr670Ile-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 544-976) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Asp816Tyr-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Asp816Val-Mutant) (Active) protein (GST tag)KIT 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (Thr670Ile-Mutant) (Active) protein (His-GST)KIT 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 544-976) (Active) protein (His-GST)KIT 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (Fc Tag)KIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 1-976) protein (GST tag)KIT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
- 别名
- KIT (KIT 产品)
- 别名
- C-Kit Protein, CD117 Protein, PBT Protein, SCFR Protein, Bs Protein, Fdc Protein, Gsfsco1 Protein, Gsfsco5 Protein, Gsfsow3 Protein, SCO1 Protein, SCO5 Protein, SOW3 Protein, Ssm Protein, Tr-kit Protein, W Protein, c-KIT Protein, c-kit Protein, MGF Protein, KIT Protein, C-KIT Protein, kit Protein, receptor Protein, sparse Protein, Xkl-1 Protein, kit-a Protein, kit-b Protein, kl1-A Protein, krk1 Protein, pbt Protein, scfr Protein, KIT proto-oncogene receptor tyrosine kinase Protein, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Protein, KIT proto-oncogene receptor tyrosine kinase S homeolog Protein, KIT Protein, Kit Protein, kita Protein, kit Protein, kit.S Protein
- 背景
- Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. {ECO:0000269|PubMed:10397721, ECO:0000269|PubMed:12444928, ECO:0000269|PubMed:12511554, ECO:0000269|PubMed:12878163, ECO:0000269|PubMed:17904548, ECO:0000269|PubMed:19265199, ECO:0000269|PubMed:21135090, ECO:0000269|PubMed:21640708, ECO:0000269|PubMed:7520444, ECO:0000269|PubMed:9528781}.
- 分子量
- 108.3 kDa Including tag.
- UniProt
- P10721
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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