电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

E2F8 Protein (AA 1-867) (His tag)

Crystallography grade E2F8 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
产品编号 ABIN3092279
发货至: 中国
  • 抗原 See all E2F8 蛋白
    E2F8 (E2F Transcription Factor 8 (E2F8))
    蛋白类型
    Recombinant
    产品特性
    AA 1-867
    宿主
    • 1
    • 1
    资源
    • 1
    • 1
    Insect Cells
    标记
    This E2F8 protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    序列
    MENEKENLFC EPHKRGLMKT PLKESTTANI VLAEIQPDFG PLTTPTKPKE GSQGEPWTPT ANLKMLISAV SPEIRNRDQK RGLFDNRSGL PEAKDCIHEH LSGDEFEKSQ PSRKEKSLGL LCHKFLARYP NYPNPAVNND ICLDEVAEEL NVERRRIYDI VNVLESLHMV SRLAKNRYTW HGRHNLNKTL GTLKSIGEEN KYAEQIMMIK KKEYEQEFDF IKSYSIEDHI IKSNTGPNGH PDMCFVELPG VEFRAASVNS RKDKSLRVMS QKFVMLFLVS TPQIVSLEVA AKILIGEDHV EDLDKSKFKT KIRRLYDIAN VLSSLDLIKK VHVTEERGRK PAFKWTGPEI SPNTSGSSPV IHFTPSDLEV RRSSKENCAK NLFSTRGKPN FTRHPSLIKL VKSIESDRRK INSAPSSPIK TNKAESSQNS APFPSKMAQL AAICKMQLEE QSSESRQKVK VQLARSGPCK PVAPLDPPVN AEMELTAPSL IQPLGMVPLI PSPLSSAVPL ILPQAPSGPS YAIYLQPTQA HQSVTPPQGL SPTVCTTHSS KATGSKDSTD ATTEKAANDT SKASASTRPG SLLPAPERQG AKSRTREPAG ERGSKRASML EDSGSKKKFK EDLKGLENVS ATLFPSGYLI PLTQCSSLGA ESILSGKENS SALSPNHRIY SSPIAGVIPV TSSELTAVNF PSFHVTPLKL MVSPTSVAAV PVGNSPALAS SHPVPIQNPS SAIVNFTLQH LGLISPNVQL SASPGSGIVP VSPRIESVNV APENAGTQQG RATNYDSPVP GQSQPNGQSV AVTGAQQPVP VTPKGSQLVA ESFFRTPGGP TKPTSSSCMD FEGANKTSLG TLFVPQRKLE VSTEDVH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human E2F8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Protein is endotoxin free.
    质量等级
    Crystallography grade
    Top Product
    Discover our top product E2F8 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    E2F8 (E2F Transcription Factor 8 (E2F8))
    别名
    E2F8 (E2F8 产品)
    别名
    MGC130750 Protein, E2F8 Protein, E2F-8 Protein, RGD1308091 Protein, 4432406C08Rik Protein, AA410048 Protein, E2F transcription factor 8 Protein, E2F transcription factor 8 L homeolog Protein, E2F8 Protein, e2f8.L Protein, e2f8 Protein, E2f8 Protein
    背景
    Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. {ECO:0000269|PubMed:15897886, ECO:0000269|PubMed:16179649, ECO:0000269|PubMed:18202719, ECO:0000269|PubMed:22903062}.
    分子量
    95.1 kDa Including tag.
    UniProt
    A0AVK6
You are here:
客服