Misato Homolog 1 (MSTO1) (AA 1-570) protein (His tag)

ABIN3084029 产品详细信息, 供应商: Log in to see
蛋白名称
  • BC008103
  • mst
  • MST
  • misato
  • wu:fi40a10
  • zgc:55470
  • misato homolog 1 (Drosophila)
  • misato 1, mitochondrial distribution and morphology regulator
  • Msto1
  • MSTO1
  • msto1
产品特性
AA 1-570
1
1
1
1
宿主

2
1
资源
Insect Cells
1
1
1
蛋白类型
Recombinant
标记
His tag
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 MAGGAREVLT LQLGHFAGFV GAHWWNQQDA ALGRATDSKE PPGELCPDVL YRTGRTLHGQ ETYTPRLILM DLKGSLSSLK EEGGLYRDKQ LDAAIAWQGK LTTHKEELYP KNPYLQDFLS AEGVLSSDGV WRVKSIPNGK GSSPLPTATT PKPLIPTEAS IRVWSDFLRV HLHPRSICMI QKYNHDGEAG RLEAFGQGES VLKEPKYQEE LEDRLHFYVE ECDYLQGFQI LCDLHDGFSG VGAKAAELLQ DEYSGRGIIT WGLLPGPYHR GEAQRNIYRL LNTAFGLVHL TAHSSLVCPL SLGGSLGLRP EPPVSFPYLH YDATLPFHCS AILATALDTV TVPYRLCSSP VSMVHLADML SFCGKKVVTA GAIIPFPLAP GQSLPDSLMQ FGGATPWTPL SACGEPSGTR CFAQSVVLRG IDRACHTSQL TPGTPPPSAL HACTTGEEIL AQYLQQQQPG VMSSSHLLLT PCRVAPPYPH LFSSCSPPGM VLDGSPKGAA VESIPVFGAL CSSSSLHQTL EALARDLTKL DLRRWASFMD AGVEHDDVAE LLQELQSLAQ CYQGGDSLVD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human MSTO1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin free.
质量等级 Crystallography grade
背景 Involved in the regulation of mitochondrial distribution and morphology. {ECO:0000269|PubMed:17349998}.
分子量 62.8 kDa Including tag.
UniProt Q9BUK6
研究领域 Signaling, Metabolism
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
说明

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Misato Homolog 1 (MSTO1) (AA 1-570) protein (His tag) (ABIN3084029) „Crystallography Grade“ protein due to multi-step, protein-specific purification process