Sulfotransferase Family 1B Member 1 (SULT1B1) (AA 1-296) protein (His tag)

ABIN3077337 产品详细信息, 供应商: Log in to see
蛋白名称
  • ST1B1
  • ST1B2
  • SULT1B2
  • St2b2
  • SULT1B
  • cSULT1B1
  • MGC80677
  • sulfotransferase family, cytosolic, 1B, member 1
  • sulfotransferase family 1B, member 1
  • sulfotransferase family, cytosolic, 1D, member 1
  • SULT1B1
  • Sult1b1
  • sult1b1
  • SULT1D1
产品特性
AA 1-296
6
2
1
1
1
1
宿主

9
1
1
1
1
1
资源
大肠杆菌(E. Coli)
7
4
2
1
蛋白类型
Recombinant
标记
This SULT1B1 protein is labelled with His tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 MLSPKDILRK DLKLVHGYPM TCAFASNWEK IEQFHSRPDD IVIATYPKSG TTWVSEIIDM ILNDGDIEKC KRGFITEKVP MLEMTLPGLR TSGIEQLEKN PSPRIVKTHL PTDLLPKSFW ENNCKMIYLA RNAKDVSVSY YHFDLMNNLQ PFPGTWEEYL EKFLTGKVAY GSWFTHVKNW WKKKEEHPIL FLYYEDMKEN PKEEIKKIIR FLEKNLNDEI LDRIIHHTSF EVMKDNPLVN YTHLPTTVMD HSKSPFMRKG TAGDWKNYFT VAQNEKFDAI YETEMSKTAL QFRTEI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human SULT1B1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Endotoxin has not been removed. Please contact us if you require endotoxin removal.
质量等级 Crystallography grade
背景 Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine, reverse triiodothyronine and thyroxine. {ECO:0000269|PubMed:9443824, ECO:0000269|PubMed:9463486}.
分子量 35.9 kDa Including tag.
UniProt O43704
研究领域 Signaling, Hormones, Metabolism
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
说明

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Sulfotransferase Family 1B Member 1 (SULT1B1) (AA 1-296) protein (His tag) (ABIN3077337) „Crystallography Grade“ protein due to multi-step, protein-specific purification process