X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 2 (XRCC2) (AA 1-280) protein (His tag)

ABIN3075640 产品详细信息, 供应商: Log in to see
蛋白名称
  • XRCC2
  • 4921524O04Rik
  • 8030409M04Rik
  • RAD51
  • RecA
  • RGD1564823
  • X-ray repair complementing defective repair in Chinese hamster cells 2
  • XRCC2
  • LOC100218547
  • Xrcc2
产品特性
AA 1-280
1
1
宿主

3
资源
Insect Cells
1
1
1
蛋白类型
Recombinant
标记
This XRCC2 protein is labelled with His tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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序列 MCSAFHRAES GTELLARLEG RSSLKEIEPN LFADEDSPVH GDILEFHGPE GTGKTEMLYH LTARCILPKS EGGLEVEVLF IDTDYHFDML RLVTILEHRL SQSSEEIIKY CLGRFFLVYC SSSTHLLLTL YSLESMFCSH PSLCLLILDS LSAFYWIDRV NGGESVNLQE STLRKCSQCL EKLVNDYRLV LFATTQTIMQ KASSSSEEPS HASRRLCDVD IDYRPYLCKA WQQLVKHRMF FSKQDDSQSS NQFSLVSRCL KSNSLKKHFF IIGESGVEFC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human XRCC2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Protein is endotoxin free.
质量等级 Crystallography grade
背景 Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. {ECO:0000269|PubMed:11751635, ECO:0000269|PubMed:11834724, ECO:0000269|PubMed:21276791, ECO:0000269|PubMed:23149936}.
分子量 32.9 kDa Including tag.
UniProt O43543
研究领域 DNA/RNA, Chromatin and Nuclear Signaling
途径 DNA Damage Repair
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
说明

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 2 (XRCC2) (AA 1-280) protein (His tag) (ABIN3075640) „Crystallography Grade“ protein due to multi-step, protein-specific purification process