Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human TNF-beta / Lymphotoxin-alpha (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
LTA
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
SDS, Func
Active
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. [UniProtKB/Swiss-Prot Function]