PAK4 Protein (Transcript Variant 1)
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- 抗原 See all PAK4 蛋白
- PAK4 (P21-Activated Kinase 4 (PAK4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human PAK4 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 90 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Specific activity was determined as 5,595 pmoles/min/μg, according to the Zlyte assay protocol
- Top Product
- Discover our top product PAK4 蛋白
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P21-Activated Kinase 4 (PAK4) (AA 1-591) (Active) protein (GST tag)
PAK4 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 300-591) (Active) proteinPAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 1-591) (Active) protein (His-GST)PAK4 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
P21-Activated Kinase 4 (PAK4) (AA 1-259) protein (His tag)PAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
P21-Activated Kinase 4 (PAK4) (AA 68-157) protein (GST tag)PAK4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)PAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (Strep Tag)Crystallography grade PAK4 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
P21-Activated Kinase 4 (PAK4) (AA 1-593) protein (Strep Tag)Crystallography grade PAK4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- PAK4 (P21-Activated Kinase 4 (PAK4))
- 别名
- Pak4 (PAK4 产品)
- 别名
- 5730488L07Rik Protein, AW555722 Protein, mKIAA1142 Protein, im:5916170 Protein, wu:fd12b01 Protein, zgc:92014 Protein, p21 (RAC1) activated kinase 4 Protein, p21 protein (Cdc42/Rac)-activated kinase 4 Protein, PAK4 Protein, Pak4 Protein, pak4 Protein
- 背景
- PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- 分子量
- 33.3 kDa
- NCBI登录号
- NP_005875
- 途径
- RTK signaling
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