NCF4 Protein (His tag)
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- 抗原 See all NCF4 蛋白
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This NCF4 protein is labelled with His tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human NCF-4 (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NCF4 蛋白
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the N-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))
- 别名
- Ncf-4 (NCF4 产品)
- 别名
- p40phox Protein, AI451400 Protein, NCF Protein, P40PHOX Protein, SH3PXD4 Protein, neutrophil cytosolic factor 4 Protein, ncf4 Protein, Ncf4 Protein, NCF4 Protein
- 背景
- The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
- 分子量
- 38.8 kDa
- NCBI登录号
- NP_038202
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