BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
CHO Cells
应用范围
Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human CD257 / BAFF protein expressed in CHO cells.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % , as determined by Coomassie stained SDS-PAGE.
内毒素水平
Less than 1 EU per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, determined by the dose dependent stimulation of B cells proliferation.
TNFSF13B
宿主: 人
宿主: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
应用备注
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
浓度
> 50 μg/mL
缓冲液
1 x PBS, pH 6.5
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))