Podoplanin (PDPN) (AA 12-137), (C-Term) (Active) protein (Fc Tag)

ABIN2666857 产品详细信息, 供应商: Log in to see
蛋白名称
  • PDPN
  • AGGRUS
  • GP36
  • GP40
  • Gp38
  • HT1A-1
  • OTS8
  • PA2.26
  • T1A
  • T1A-2
  • OTS-8
  • RANDAM-2
  • T1-alpha
  • T1a
  • T1alpha
  • E11
  • RTI40
  • podoplanin
  • podoplanin-like
  • PDPN
  • Pdpn
  • LOC100738269
产品特性
AA 12-137, C-Term
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
宿主
小鼠
16
8
3
1
1
资源
HEK-293
11
3
3
2
2
2
1
1
1
蛋白类型
Recombinant
生物活性
Active
标记
This Podoplanin protein is labelled with Fc Tag.
应用范围
Flow Cytometry (FACS)
选项
Supplier
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Supplier Product No.
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纯度 > 95 % , as determined by Coomassie stained SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平

Less than 0.1 EU per μg of protein as determine by the LAL method.

背景 Podoplanin, also termed GP38, is a 38 kD type I transmembrane sialoglycoprotein and a member of the podoplanin family. Mouse and human GP38 share 46 % amino acid sequence homology. Alternative splicing transcripts, encoding different isoforms of GP38, have been identified. Structurally, GP38 has a 119 amino acid extracellular domain (ECD), a 21 amino acid transmembrane region, and a 10 amino acid cytoplasmic tail. This type I protein is heavily O-glycosylated and has a diverse distribution in human tissues, including glomerular epithelial cells, lung alveolar cells, lymphatic endothelial cells, and neural tissues. GP38 is also expressed by tumor cells such as testicular seminomas, squamous cell carcinomas, colorectal tumors, and brain tumors. Therefore, this protein is a target of antibody therapy in cancer treatment. GP38 induces platelet aggregation and tumor metastasis through its association with CLEC2. The binding of GP38 and CLEC2 is important for maintaining high endothelial cell venule integrity. Also, GP38 is necessary for lymphatic vessel formation, normal lung cell proliferation, and alveolus formation at birth. In addition, GP38 was shown to be a novel marker for immature hepatic progenitor cells in fetal livers. GP38 is upregulated in Th17 cells in the arthritic joints in SKG mice, suggesting that this protein could be a useful Th17 cell marker for diagnosing pathological conditions of autoimmune diseases, including rheumatoid arthritis.
分子量 This 362 amino acid recombinant protein predicted has a molecular mass of approximately 39.8 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 130 kDa in non-reducing conditions. The predicted N-terminal amino acid
途径 Dicarboxylic Acid Transport
应用备注 Optimal working dilution should be determined by the investigator.
说明

Biological activity: Recombinant mouse podoplanin, when coated on a plate (2 μg/ml, 100 μl/well), is able to bind mouse CLEC-2 in a dose dependent manner with an apparent kD <1.0 nM.

限制 仅限研究用
状态 Liquid
溶解方式 For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
浓度 200 μg/mL
缓冲液 0.22 μm filtered protein solution is in PBS.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 -20 °C
储存方法 Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
厂商提供的图像
ELISA image for Podoplanin (PDPN) (AA 12-137), (C-Term) (Active) protein (Fc Tag) (ABIN2666857) Podoplanin (PDPN) (AA 12-137), (C-Term) (Active) protein (Fc Tag)
背景 Miyamoto, Uga, Tanaka, Kadowaki, Ikeda, Saegusa, Morinobu, Kumagai, Kurata: "Podoplanin is an inflammatory protein upregulated in Th17 cells in SKG arthritic joints." in: Molecular immunology, Vol. 54, Issue 2, pp. 199-207, 2013 (PubMed).

Konishi, Yasuchika, Ishii, Fukumitsu, Kamo, Fujita, Ikai, Uemoto: "A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers." in: In vitro cellular & developmental biology. Animal, Vol. 47, Issue 1, pp. 45-53, 2011 (PubMed).

Suzuki-Inoue, Kato, Inoue, Kaneko, Mishima, Yatomi, Yamazaki, Narimatsu, Ozaki: "Involvement of the snake toxin receptor CLEC-2, in podoplanin-mediated platelet activation, by cancer cells." in: The Journal of biological chemistry, Vol. 282, Issue 36, pp. 25993-6001, 2007 (PubMed).

Mishima, Kato, Kaneko, Nishikawa, Hirose, Matsutani: "Increased expression of podoplanin in malignant astrocytic tumors as a novel molecular marker of malignant progression." in: Acta neuropathologica, Vol. 111, Issue 5, pp. 483-8, 2006 (PubMed).

Schacht, Dadras, Johnson, Jackson, Hong, Detmar: "Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors." in: The American journal of pathology, Vol. 166, Issue 3, pp. 913-21, 2005 (PubMed).

Kato, Fujita, Kunita, Sato, Kaneko, Osawa, Tsuruo: "Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51599-605, 2003 (PubMed).

Williams, Cao, Hinds, Rishi, Wetterwald: "T1 alpha protein is developmentally regulated and expressed by alveolar type I cells, choroid plexus, and ciliary epithelia of adult rats." in: American journal of respiratory cell and molecular biology, Vol. 14, Issue 6, pp. 577-85, 1996 (PubMed).

Farr, Nelson, Hosier: "Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 40, Issue 5, pp. 651-64, 1992 (PubMed).