Neuregulin 1 Protein (NRG1) (AA 176-246)
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- 抗原 See all Neuregulin 1 (NRG1) 蛋白
- Neuregulin 1 (NRG1)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 176-246
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Neuregulin 1 (NRG1) protein (Fc Tag)
NRG1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Neuregulin 1 (NRG1) (AA 119-237) protein (His-IF2DI Tag)NRG1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Neuregulin 1 (NRG1) (AA 1-241) protein (GST tag)NRG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Neuregulin 1 (NRG1) (AA 20-242) protein (His tag)Crystallography grade NRG1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Neuregulin 1 (NRG1) (AA 266-640) protein (His tag)Crystallography grade NRG1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Neuregulin 1 (NRG1) (AA 20-241) protein (rho-1D4 tag)Crystallography grade NRG1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Neuregulin 1 (NRG1) (AA 20-640) protein (rho-1D4 tag)Crystallography grade NRG1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 = 0.2 - 1 ng/ml, corresponding to a specific activity of 1 - 5 x 106 units/mg, as determined by induction of MCF-7 cell proliferation.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- Neuregulin 1 (NRG1)
- 别名
- NRG1 (NRG1 产品)
- 别名
- 6030402G23Rik Protein, ARIA Protein, D230005F13Rik Protein, GGF Protein, GGFII Protein, HRG Protein, HRGalpha Protein, Hgl Protein, NDF Protein, SMDF Protein, GGF2 Protein, HGL Protein, HRG1 Protein, HRGA Protein, MST131 Protein, NRG-1 Protein, heregulin Protein, nrg1-A Protein, pro-neuregulin-1 Protein, NRG1 Protein, wu:fm70e01 Protein, DKFZp459A1838 Protein, neuregulin 1 Protein, histidine rich glycoprotein Protein, neuregulin 1 L homeolog Protein, Nrg1 Protein, NRG1 Protein, HRG Protein, nrg1.L Protein, nrg1 Protein
- 背景
- Neuregulin 1 (NRG1) was initially identified in the conditioned medium of a human breast tumor cell line as a protein of 45 kD, which induces the phosphorylation of a tyrosine kinase (ERBB2). The neuregulin family includes four members (NRG1-NRG4) that are encoded from four individual genes. Through differential promoter usage and splicing, these members generate 12 isoforms. The best characterized is neuregulin 1, which includes eight isoforms, all isoforms contain (from N-terminus to C-terminus) an immunoglobulin-like (Ig) domain, a growth factor-like domain (EGF), a transmembrane (TM), and a cytoplasmic domain. Alternative splicing in the EGF-like domain of neuregulin 1 results in α and β isoforms. The EGF-like domain is necessary and sufficient for neuregulin bioactivity. The EGFR family of receptors includes four members (ErbB1 through ErbB4), and neuregulins bind to the ErbB3 and ErbB4 receptors. Neuregulins are released from the membrane by proteolytic cleavage, and this process is required for the binding of the neuregulin derived fragments to ErbB receptors. The neuregulin1-β isoform is predominant in the central nervous system and participates in development, survival, and metabolism in neuron and glial cells. Neuregulin1-β is neuroprotective and attenuates inflammatory responses induced by ischemic stroke in rats, preventing macrophage and microglial infiltration and astrocytic activation. Also, neuregulin1-β blocks the induction of pro-inflammatory and stress genes provoked by ischemia. Neuregulins 1-4 are expressed in approximately 25 % of breast cancer carcinomas and increase breast cancer cell proliferation, increase tumorigenesis, and promote invasive characteristics of cancer cells. In this sense, neuregulin1-β induces MMP-1 and MMP-9 in cancer cell lines.
- 分子量
- The 71 amino acid recombinant protein has a predicted molecular mass of approximately 8.2 kDa. The DTT-reduced and non-reduced protein migrate at approximately 11 kDa and 12 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Thr.
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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